· Compulsory/optional course: optional
· The Doctoral Programme in Biomedicine coordinates the course
· The course is part of the Field-Specific Studies module
· The course is available to students of other degree programmes
The aim is to open up the field of in vivo animal imaging and its use in non/pre-clinical applications. The course is useful for students who might benefit from using in vivo animal imaging for their own research.
The course will cover a range of current methods for in vivo animal imaging, with an emphasis on those that are available for scientists within HiLife/UH, and focusing on recent and8scientifically exciting applications.
Date: Tue 17.12. 2019
Time: 09:00 - 16:00 Biomedicum
Venue: Lecture Hall 3, Haartmaninkatu 8
Date: December 18 - early January 2020, depending on the number of participants, see below.
Time: 10-14 depending on the number of participants. Approx. 2 hours morning/afternoon; times will be assigned depending on the number of participants.
Venue: Meilahti/Viikki Campus
Minisymposium (Tuesday December 17, 2019. Open to all). The lectures cover the principles of in vivo animal imaging modalities including intravital imaging, optical small animal imaging, microCT and PET/SPECT/CT, and practicalities of their use at UH, as well as examples of in vivo imaging research on cancer, neurobiology, drug development and cardiovascular diseases. Lectures are open for everyone.
Demonstrations (times to be agreed according to participant numbers, NB! Intravital imaging using a cranial window and SPECT/CT will be organized 18.12.2019): Students may choose 1-2 in vivo animal imaging modalities for demos using UH imaging facilities, including intravital multiphoton imaging of dermal vasculature and/or cranial window), SPECT/CT, microCT imaging (bones, lungs) or luminescence/fluorescence small animal imaging. NB! The number of participants in the demonstrations is limited.
- A single day of lectures, open to everyone on Tuesday December 17, 2019, 09:00 - 16:00
- Participation in in vivo imaging demonstrations (times to be announced).
Choi et al., In Vivo Fluorescence Microscopy: Lessons from Observing Cell Behavior in Their Native Environment. PHYSIOLOGY 30: 40–49, 2015; doi:10.1152/physiol.00019.2014
Secklehner et al., Intravital microscopy in historic and contemporary immunology. Immunol Cell Biol 95(6): 506–513, 2017; doi: 10.1038/icb.2017.25.
Conway et al., Context-dependent intravital imaging of therapeutic response using intramolecular FRET biosensors. Methods. 28:78-94 2017. doi: 10.1016/j.ymeth.2017.04.014.
de Jong et al., Imaging preclinical tumour models: improving translational power. Nat Rev Cancer 14(7):481-93, 2014; doi: 10.1038/nrc3751.
Bernsen et al., Biomarkers in preclinical cancer imaging. Eur J Nucl Med Mol Imaging 42:579–596 2015; doi: 10.1007/s00259-014-2980-7
Clark and Badea, Micro-CT of rodents: State-of-the-art and future perspectives. Phys Med. 6:619-34 2014; doi: 10.1016/j.ejmp.2014.05.011
Intravital microscopic imaging of tumors: https://www.jove.com/video/51916/real-time-imaging-myeloid-cells-dynamics-apcmin-intestinal-tumors (Time 4:57)
Intravital microscopic imaging of inflammation: http://www.jove.com/video/53585/imaging-cd4-t-cell-interstitial-migration-in-the-inflamed-dermisTime (Time 10:12)
Intravital microscopic imaging of vascular permeability
PET/CT Imaging of brown adipose tissue activity: https://www.jove.com/video/4060/functional-imaging-of-brown-fat-in-mice-with-18f-fdg-micro-petct (Time 10:06)
PET/CT Imaging of metabolic activity of lung tumors: https://www.jove.com/video/57167/utilizing-18f-fdg-petct-imaging-quantitative-histology-to-measure (Time 6:16)
Optical whole-body imaging of tumor growth: https://www.jove.com/video/1210/in-vivo-bioluminescent-imaging-of-mammary-tumors-using-ivis-spectrum (Time 7:51)
microCT imaging of lung tumors: https://www.jove.com/video/53904/using-micro-computed-tomography-for-assessment-tumor-development (Time 10:15)
Exam or homeworks, reading, a written assignment.
Pass / fail
Mini-symposium (full attendance), pre-assignment (obligatory), demonstrations (optional), research plan (optional)
1 cr: pre-assignment + full attendance at lectures OR
2 cr: pre-assignment + full attendance at lectures + participation in 1 demonstration + research plan OR
2 cr: pre-assignment + full attendance at lectures + participation in 2 demonstrations
Pre-assignment. Choose two review articles and three of the movies below. Based on these, write three questions about in vivo imaging topics that you may want to know more about, or that remain unclear to you. The questions should be submitted by email, but in addition you can ask them at the minisymposium after any relevant lecture. The task is to be completed before the course, by December 13th, 2019.
Research plan for in vivo imaging. Write a 0.5 page “research plan” how you could or would like to utilize in vivo imaging in your research. The plan may involve the use of one or several of the in vivo modalities. The task is to be completed before or after the course, latest by December 18th, 2019, and submitted as an email attachment.
The assignments are to be submitted via Moodle. Information provided for registered participants. The course material is listed below.
Lectures are open to all, but registration via e-lomake is required for all (by 3.12.2019), to arrange sufficient refreshments for the coffee break, and for participation in demos: https://elomake.helsinki.fi/lomakkeet/101883/lomake.html
Doctoral candidates and students should also enroll via WebOodi: https://courses.helsinki.fi/en/dpbm-148/131042615
Note that UH graduate students will take precedence in demonstrations if spaces fill up.
Feedback form: https://elomake.helsinki.fi/lomakkeet/99648/lomake.html
One-day open minisymposium with lectures + a choice of 2-3 demos during two days.