- The course belongs to Chemistry and Molecular Science master program.
- The course is compulsory in the Analysis and Separation module.
- This master level course is also available to interested students from other degree programmes.
- Basic knowledge of separation techniques (gas and liquid chromatography).
- Basic knowledge of mass-spectrometric techniques.
- Basic knowledge of the physical chemistry of solution-phase equilibria, especially the following aspects: acid/base theory; pH; buffers; dissociation constants.
- Basic understanding of organic chemistry (structure; functional groups; functional group transformation).
Students attending this course will know:
- Crucial role of sampling and sample preparation in the workflow fo analytical sciences.
- Basic aspects of sampling theory, types of sampling errors, and countermeasures.
- Basics sampling procedures for solids, liquids, semivolatiles and gases.
- Potential sources of sample contamination/sample loss and countermeasures.
- Drying procedures. Methods for determination of water content and residual solvents.
- Physical/chemical principles of various sample extraction/enrichment techniques (Extraction; Filtration; Sedimentation; Membrane Separation) and the scope of applications to various sample preparation challenges.
- Dedicated calibration approaches to facilitate the determination of analyte recoveries.
- Analyte derivatization strategies for gas and liquid chromatographic, and electrophoretic applications, including hyphenated separation techniques, such as LC-MS and GC-MS.
First/second year of master studies.
Autumn term, Period II
This course aims at providing students with a thorough understanding of the crucial role of sampling and sample preparation techniques within the workflow of analytical sciences. The topics covered will include a basic introduction into the fundamentals of Sample Theory, the types of sampling errors and potential measures to avoid the latter; the basic procedures employed for sampling solid, liquid, semivolatile and gaseous matter; and the potential sources of and countermeasures against sample contamination/sample loss. Sample drying procedures and methods for the assessment of residual water/solvent will be discussed. This will be followed by a comprehensive overview of current techniques for sample extraction/enrichment (various extraction; filtration; sedimentation; membrane separation, and chromatographic techniques) will be given, along with an in-depth discussions of their scope of applications, and merits and limitations. Important approaches for the convenient determination of analyte recovery from samples with complex matrices will be outlined. Finally, the use of dedicated analyte derivatization strategies in enabling and/or facilitating analytical protocols will be discussed, with special emphasis being placed on modern hyphenated separation techniques, such as LC-MS and GC-MS.
Information will be added and up-dated in due course.
- Student group seminars on topic-relevant research papers from the recent literature. In this exercise, a group of students will prepare and give a presentation on the selected paper, while another group of students will engage the presenters in a critical discussion. This exercise is an integral part of the lecture course and contributes to the overall credits achievable.
- Possibly, excursions to certain labs at the campus for at-site demonstration/discussion of instrumentation and/or techniques.
Written exam (75% of total credit).
Student group seminar presentation & discussion exercise (25% of total credit).
Grading scale from 0-5, with 5 being top grading and 0 being failed.
Contact teaching. Note that regular attendance of the lecture course (>80%) is strongly encouraged and expected.