Not compulsory, recommended for students in Master's Programme in Microbiology and Microbial Biotechnology, available for students in other program if places are available
Basic laboratory skills achieved during practical courses in bachelor level studies. Basic understanding of biochemistry and microbiology achieved during bachelor level studies. Lecture course in metabolism is highly recommended as a preceding course.
Many courses in microbiology and biochemistry.
The student will learn to plan and evaluate experimental set-ups, as the whole course is conceived as an experiment, where the yeast growth conditions will first be selected, then the experiment performed and finally the results compared. The student will learn the basic principles of the use of yeast as a versatile experimental organism, as well as yeast genetics. The student will understand the basic ideas in metabolic engineering.
During master degree studies, suitable for 1st year master degree students
Arranged every second year, even years.
We will grow metabolically modified yeast strains and analyze cell growth as well as the production of some basic and derived (engineered) metabolites. Yeast transformation and basic concepts of yeast genetics as well as the use of auxotrophic selection markers will be included.
Laboratory protocols and reading material (e.g. scientific articles) provided by the teacher.
The teacher will introduce the concepts and methods used through short lectures and demonstrations. Supportive reading and other material as well as web links will be provided through Moodle.
The students will participate in the planning and selection of the growth conditions to be used by each person/group in the preliminary planning section, so that the effect of different parameters may be analyzed. The students will plan their own laboratory schedules. The students will keep careful lab notes of the work performed. All the results will be compiled and compared at the concluding feedback session.
Evaluated on the scale 0–5. Evaluation is based on the report written by the student
Moodle will be utilized
The course consists of three weeks of laboratory work, and will additionally include a prior planning session and a later, concluding feedback session. The students will write a report during/after the course.
Replaces the former course 85069 (BKEM201).