Optional course for Master students in Pharmacy, specifically for those intending to make a pro gradu project applying the studied techniques
A student entering the course should have good basic knowledge of Biochemistry and Cell and Molecular Biology (e.g. Principles of Bioscience in Pharmacy, or similar studies).
After completing the course, the student should have basic knowledge and skills in experimental cell and molecular biology methods that are applied in different disciplines within Pharmacy. More than providing theoretical knowledge on the principles of the applied techniques, the course aims at giving sufficient practical skills for starting an experimental pro gradu thesis project that involve cell-based in vitro methods. In addition, the course will provide an opportunity to focus in one of few more specific areas and the select will be able to select among them.
4th year spring semester, period 3
The methods and techniques that will be covered within the course include cell culture and cell viability assays, cloning and PCR, gene expression analysis with reporter gene-based constructs, as well as protein expression analysis by Western blotting and ELISA. The more specific topics, from which the student could choose one, include primary cell isolation, binding and functional studies of pharmacological agents, viral infections of animal cell cultures and protein expression and purification in bacterial cells.
Article from scientific literature and textbooks chapters. To be announced during the course
Scale 0-5. The final grade will be based on the lab work and related reports (80%) and on the group presentation (20%).
The groups and teaching times for the laboratory work will be informed in the beginning of the course. The attendance in the laboratory work is mandatory and working hours will be within 8-16 during the teaching period.
Pre-course exam, lectures (12 h), lab work (80 h), writing working plans and reports on lab work (35 h), a group presentation.